Laboratory Linearity

As with all immunoassays, Freelite assays are potentially prone to sample non-linearity.

When samples give different values at different dilutions but are not in antigen excess we refer to the sample as being ‘non-linear’.

Non-linearity with the Freelite assay may be due to the properties of a particular monoclonal free light chain or may be caused by non-specific assay interference (e.g. the presence of lipids).

For a consistent approach, we recommend using the dilutions stated in the Freelite product inserts. If such an approach is used, sample non-linearity will be minimised.