Subclasses Laboratory Information
The Optilite system has intelligent scheduling to help streamline your laboratory’s workflow and ensure faster test turnaround times. Each IgG subclass assay has been optimised to enhance your productivity:
- Automatic re-dilution to end result ensures no additional manual dilutions are required
- Wide measuring ranges with large dilution steps minimises reagent usage
The efficiency of our IgG subclass assays was compared to alternative reagents / platforms.
An identical set of samples were run on Optilite, SPAPLUS, and two other commercially available analysers using reagents from the respective analyser manufacturers.
The data demonstrated that Optilite is:
- ~50% faster Analyser 1
- ~33% faster Analyser 2
* Data for the SPAPLUS and Analyser 2 does not include the time required to perform manual dilutions
IgG subclass calibration to reference standard ERM-DA470k
- ERM-DA470k is accepted by the International Federation of Clinical Chemistry (IFCC)
- Alternative IgG subclass assays are calibrated to the World Health Organisation standard
- WHO67/97 (Sanquin M1590). Measuring IgG3 and IgG4 concentrations in WHO 67/97 have shown considerable laboratory to laboratory variability.3,4,5
References
1.) Bossuyt, X., et al., Determination of IgG subclasses: a need for standardization. J Allergy Clin Immunol, 2005. 115(4): p. 872-874.
2.) Bernasconi, L., et al., Variable and inaccurate serum IgG4 levels resulting from lack of standardization in IgG subclass assay calibration. Clin Chem Lab Med, 2019.
3.) Klein, F., et al., The quantification of human IgG subclasses in reference preparations. Clin Chim Acta, 1985. 150(2): p. 119-127.